Analyzing T Cell Responses PDF
By:Dirk Nagorsen,Francesco M. Marincola
Published on 2006-01-16 by Springer Science & Business Media
Active specific immunotherapy is a promising but investigational modality in the management of cancer patients. Currently, several different cancer vaccine formulations such as peptides, proteins, antigen-pulsed dendritic cells, whole tumor cells, etc. in combination with various adjuvants and carriers are being evaluated in clinical trials (1-3). To determine the optimal cancer vaccine strategy, a surrogate immunological end-point that correlates with clinical outcome needs to be defined, since it would facilitate the rapid comparison of these various formulations. Traditional immunological assays such as ELISA, proliferation and cytotoxicity assays can detect immune responses in vaccinated patients but are not quantitative. In contrast, novel assays such as enzyme-linked immunospot (ELISPOT) assay, intracellular cytokine assay and tetramer assay can quantitate the frequency of antigen-specific T cells. Of these, the ELISPOT assay has the 5 lowest detection limit with 1/10 peripheral blood mononuclear cells (PBMC) and has been determined to be one of the most useful assays to evaluate immune response to cancer vaccines (4). However, the IFN-? ELISPOT assay is not an exclusive measure of cytotoxic T-lymphocyte (CTL) activity as non-cytotoxic cells can also secrete IFN-?. Additionally, CTL with lytic activity do not always secrete IFN-? (5). A more relevant approach to assess functional activity of cytotoxic lymphocytes would be to measure the secretion of molecules that are associated with lytic activity. One of the major mechanisms of cell-mediated cytotoxicity involves exocytosis of cytoplasmic granules from the effector toward the target cell.
This Book was ranked at 30 by Google Books for keyword epidemiology triangle influenza.
Book ID of Analyzing T Cell Responses's Books is QwaeLnDeFtQC, Book which was written byDirk Nagorsen,Francesco M. Marincolahave ETAG "ymcHctcjqhU"
Book which was published by Springer Science & Business Media since 2006-01-16 have ISBNs, ISBN 13 Code is 9781402036231 and ISBN 10 Code is 140203623X
Reading Mode in Text Status is true and Reading Mode in Image Status is true
Book which have "314 Pages" is Printed at BOOK under CategoryMedical
Book was written in en
eBook Version Availability Status at PDF is true and in ePub is true
Book Preview
Download Analyzing T Cell Responses PDF Free
Download Analyzing T Cell Responses Book Free
Download Analyzing T Cell Responses Free
Download Analyzing T Cell Responses PDF
Download Analyzing T Cell Responses Book
How to Download Analyzing T Cell Responses Book
How to Download Analyzing T Cell Responses
How to Download Analyzing T Cell Responses pdf
How to Download Analyzing T Cell Responses free
Free Download Analyzing T Cell Responses
No comments:
Post a Comment